Thermodynamic Modeling and Simulation of Aqueous Two-Phase Bioseparation Systems

Currently there is low application of process system engineering in bioengineering studies. Meanwhile, Aqueous Two-Phase Systems (ATPS) that promises significant contributions as a bioseparation technique offers better yield, more economical and there exists new strategies of modification. Hence, ATPS is a good basis to develop sophisticated design methods. Furthermore, ATPSs have not been extensively adopted in process plants due to limited understanding on mechanism, behaviors and application. This talk presents an effort to create and validate a thermodynamic framework to predict the ATPS performance that is valid for multicomponent systems. The thermodynamic model is able to predict the liquid-liquid behaviour of water-PEG6000-DxT500 system similar to that reported by a previous work by Johansson et al (1998).