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Comparative Analysis of Multiple Designs for Aqueous Two-Phase Protein Extraction Systems

Ahmad, Murni M (2006) Comparative Analysis of Multiple Designs for Aqueous Two-Phase Protein Extraction Systems. In: Annual Review Meeting (ARM 2006), February 2006, Pittsburgh, USA.

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Abstract

A comparative analysis are made to compare flowsheet designs of the application of Aqueous Two-Phase System (ATPS) to extract protein from cell lysate. The analysis that observes the protein purity and yield trends is conducted using a flowsheet calculation framework that calculates the thermodynamic behaviour of the polymers and polymer-salt systems containing protein(s) within a limited range of properties and concentration. The designs include one-stage and two-stage extraction stages containing two types of ATPS i.e. PEG6000-DxT500 and PEG6000-Na3PO4 containing phosphofructokinase and ovalbumin. The results match previous findings to some extent. The analysis highlights potential for flowsheet design optimization work.

Item Type:Conference or Workshop Item (Paper)
Subjects:T Technology > TP Chemical technology
Departments / MOR / COE:Departments > Chemical Engineering
ID Code:3232
Deposited By: Dr Murni M Ahmad
Deposited On:16 Nov 2010 04:02
Last Modified:19 Jan 2017 08:27

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