Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: An updated review

Semail, N.-F. and Abdul Keyon, A.S. and Saad, B. and Noordin, S.S. and Nik Mohamed Kamal, N.N.S. and Mohamad Zain, N.N. and Azizi, J. and Kamaruzaman, S. and Yahaya, N. (2020) Analytical method development and validation of anticancer agent, 5-fluorouracil, and its metabolites in biological matrices: An updated review. Journal of Liquid Chromatography and Related Technologies, 43 (15-16). pp. 562-579.

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Abstract

5-fluorouracil (5-FU) refers to a fluorinated pyrimidine analogue that has been widely used as an anticancer agent for colon, head, and neck cancers. Detection of 5-FU and its metabolites; 5-fluorouridine and 5-fluoro-2-deoxyuridine in biological samples allows optimization of pharmacotherapy and encourages fundamental investigations of this medication. The development of accurate and reliable sample preparation, as well as analytical methods, is critical to isolate targeted analytes from complex matrices, apart from increasing detection sensitivity of analytes. With that, this paper presents a review of prior studies pertaining to chromatographic and electrophoretic methods that focused on the analysis of 5-FU and its metabolites in biological matrices such as plasma and urine. This paper concentrates on HPLC, GC and CE systems, which are the most commonly used strategies for analytical separation of 5-FU and its metabolites from samples. Detection of these antineoplastic agents at trace level demands highly sensitive and selective analytical methodologies. Application of these analytical techniques to biological matrices is reviewed with a focus on method development strategies, including types of mobile phases and background electrolytes employed in LC and CE systems. © 2020 Taylor & Francis Group, LLC.

Item Type: Article
Impact Factor: cited By 2
Uncontrolled Keywords: Biomolecules, Analytical method development; Analytical methodology; Analytical separations; Antineoplastic agents; Back ground electrolyte; Detection sensitivity; Electrophoretic methods; Fluorinated pyrimidines, Metabolites, floxuridine; fluorouracil; fluorouridine; molecularly imprinted polymer, Article; cost effectiveness analysis; drug determination; electrophoresis; gas chromatography; high performance liquid chromatography; human; nonhuman; solid phase microextraction; tandem mass spectrometry; ultra performance liquid chromatography
Depositing User: Ms Sharifah Fahimah Saiyed Yeop
Date Deposited: 25 Mar 2022 03:14
Last Modified: 25 Mar 2022 03:14
URI: http://scholars.utp.edu.my/id/eprint/29914

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