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Thyroglobulin determination on silane�antibody functionalized interdigitated dielectrode surface to diagnose thyroid tumor

Wang, Y. and Sun, X. and Gopinath, S.C.B. and Saheed, M.S.M. and Wang, X. (2021) Thyroglobulin determination on silane�antibody functionalized interdigitated dielectrode surface to diagnose thyroid tumor. Biotechnology and Applied Biochemistry .

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Official URL: https://www.scopus.com/inward/record.uri?eid=2-s2....

Abstract

Thyroid cancer appears in endocrine glands and specific to thyroid glands has been reported widely. This work was targeted to identify and quantify thyroglobulin by using antithyroglobulin antibody complexed silane surface on interdigitated electrode (IDE) sensing surface. (3-Aminopropyl)triethoxysilane linker was used to make silane-coupling with antibody and attached on the hydroxylated IDE. This electroanalytical IDE revealed the dose-dependent responses with thyroglobulin concentrations. By getting increments with the thyroglobulin concentrations, the current responses were enhanced concomitantly and the thyroglobulin detection limit was noted as 1 pM on the linear curve y = 0.1311x + 0.5386; R² = 0.9707 with the sensitivity at lower picomolar range. Moreover, the control experiments with thyroid peroxidase and nonimmune antibody cannot yield any response of current, confirming the specific detection of thyroglobulin. This research set-up is useful to determine and quantify the thyroglobulin and diagnose thyroid cancer. © 2021 International Union of Biochemistry and Molecular Biology, Inc.

Item Type:Article
Impact Factor:cited By 0
Uncontrolled Keywords:Antibodies; Chemical detection; Diseases; Integrodifferential equations; Silanes, Control experiments; Current response; Detection limits; Endocrine glands; Interdigitated electrodes; Sensing surface; Specific detection; Thyroid peroxidase, Glycoproteins
ID Code:23907
Deposited By: Ms Sharifah Fahimah Saiyed Yeop
Deposited On:19 Aug 2021 13:24
Last Modified:19 Aug 2021 13:24

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